Pub. Date:
Springer-Verlag New York, LLC
Sexually Transmitted Diseases / Edition 1

Sexually Transmitted Diseases / Edition 1

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In recent years, molecular techniques have enhanced our ability to detect sexually transmitted infections and to conduct research to further our und- standing of sexually transmitted diseases. Molecular methods to quantitate pathogen load have also been shown to be useful for the management of HIV and other viral STDs. Existing laboratory manuals for the clinical microbi- ogy laboratory often do not include molecular methods for STDs. Sexually Transmitted Diseases: Methods and Protocols is intended to fill the need for a dedicated manual that covers all the fundamental aspects of molecular pro- cols for laboratory diagnosis, as well as research methodology for STDs, - cluding HIV. There are more than types of molecular techniques described in this book covering nine major sexually transmitted pathogens. Although mole- lar methods for the detection of such pathogens as Trichomonas vaginalis are available in the published literature, they have not been included since they are not yet widely used for laboratory diagnosis or research. Sexually Transmitted Diseases: Methods and Protocols is one of a series of books treating Methods Molecular Medicine, published by Humana Press.

Product Details

ISBN-13: 9781617370649
Publisher: Springer-Verlag New York, LLC
Publication date: 11/09/2010
Series: Methods in Molecular Medicine , #20
Edition description: Softcover reprint of hardcover 1st ed. 1999
Pages: 244
Product dimensions: 5.98(w) x 9.02(h) x 0.35(d)

Table of Contents

Part I. Overview.

The Impact of Molecular Technology on STD Control: A Historical Perspective
P. Frederick Sparling

Part II. Molecular Techniques for the Detection of Sexually Transmitted Pathogens.

Neisseria gonorrhoeae: Detection and Typing by Probe Hybridization, LCR, and PCR
Charlotte A. Gaydos and Thomas C. Quinn

Molecular Diagnosis for Chlamydia trachomatis Infections by Probe Hybridization, PCR, LCR, TMA, and Q-ß Replicase
Max A. Chernesky and James B. Mahony

Haemophilus ducreyi Detection by PCR
Patricia A. Totten, Jane Kuypers, and Stephen A. Morse

Detection of Treponema pallidum, Haemophilus ducreyi, and Herpes Simplex Virus by Multiplex PCR
Karina A. Orle and Judith B. Weiss

Detection of Genital Mycoplasmas by PCR
Claire B. Gilroy and David Taylor-Robinson

Hepatitis B Virus: Detection and Quantitation by Membrane and Liquid Hybridization, Branched DNA Signal Amplification, Hybrid Capture, and PCR Methods
Mel Krajden

Molecular Diagnosis of HIV-1 by PCR
Susan A. Fiscus

Part III. Research Techniques for Epidemiology and the Management of STDs.

Genotyping of Neisseria gonorrhoeae by Pulse Field Gel Electrophoresis and PCR
Ian W. Maclean

Genotyping Chlamydia trachomatis by PCR,Deborah Dean. Human Papillomavirus Detection by PCR and Typing by Dot-Blot
Agnetha Josefsson, Patrik Magnusson, and Ulf Gyllensten

Quantitation of HIV-1 RNA in Dried Plasma Spots (DPS): A Field Approach to Therapeutic Monitoring
Sharon A. Cassol, Francisco Diaz-Mitoma, and D. William Cameron

Collection and Processing of Seminal Plasma for the Quantitation of HIV-1 RNA by NASBA and RT-PCR
Susan A. Ficus and Myron S. Cohen

Part IV. Molecular Technology for STD Diagnosis and Research: The New Challenges.

Molecular Techniques for HIV and STDs: Implicationsfor Research and Disease Control in the New Millenium
Rosanna W. Peeling, David C. W. Mabey, and King K. Holmes


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