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In recent years, molecular techniques have enhanced our ability to detect sexually transmitted infections and to conduct research to further our und- standing of sexually transmitted diseases. Molecular methods to quantitate pathogen load have also been shown to be useful for the management of HIV and other viral STDs. Existing laboratory manuals for the clinical microbi- ogy laboratory often do not include molecular methods for STDs. Sexually Transmitted Diseases: Methods and Protocols is intended to fill the need for a dedicated manual that covers all the fundamental aspects of molecular pro- cols for laboratory diagnosis, as well as research methodology for STDs, - cluding HIV. There are more than types of molecular techniques described in this book covering nine major sexually transmitted pathogens. Although mole- lar methods for the detection of such pathogens as Trichomonas vaginalis are available in the published literature, they have not been included since they are not yet widely used for laboratory diagnosis or research. Sexually Transmitted Diseases: Methods and Protocols is one of a series of books treating Methods Molecular Medicine, published by Humana Press.
Table of ContentsPart I. Overview.
The Impact of Molecular Technology on STD Control: A Historical Perspective
P. Frederick Sparling
Part II. Molecular Techniques for the Detection of Sexually Transmitted Pathogens.
Neisseria gonorrhoeae: Detection and Typing by Probe Hybridization, LCR, and PCR
Charlotte A. Gaydos and Thomas C. Quinn
Molecular Diagnosis for Chlamydia trachomatis Infections by Probe Hybridization, PCR, LCR, TMA, and Q-ß Replicase
Max A. Chernesky and James B. Mahony
Haemophilus ducreyi Detection by PCR
Patricia A. Totten, Jane Kuypers, and Stephen A. Morse
Detection of Treponema pallidum, Haemophilus ducreyi, and Herpes Simplex Virus by Multiplex PCR
Karina A. Orle and Judith B. Weiss
Detection of Genital Mycoplasmas by PCR
Claire B. Gilroy and David Taylor-Robinson
Hepatitis B Virus: Detection and Quantitation by Membrane and Liquid Hybridization, Branched DNA Signal Amplification, Hybrid Capture, and PCR Methods
Molecular Diagnosis of HIV-1 by PCR
Susan A. Fiscus
Part III. Research Techniques for Epidemiology and the Management of STDs.
Genotyping of Neisseria gonorrhoeae by Pulse Field Gel Electrophoresis and PCR
Ian W. Maclean
Genotyping Chlamydia trachomatis by PCR,Deborah Dean. Human Papillomavirus Detection by PCR and Typing by Dot-Blot
Agnetha Josefsson, Patrik Magnusson, and Ulf Gyllensten
Quantitation of HIV-1 RNA in Dried Plasma Spots (DPS): A Field Approach to Therapeutic Monitoring
Sharon A. Cassol, Francisco Diaz-Mitoma, and D. William Cameron
Collection and Processing of Seminal Plasma for the Quantitation of HIV-1 RNA by NASBA and RT-PCR
Susan A. Ficus and Myron S. Cohen
Part IV. Molecular Technology for STD Diagnosis and Research: The New Challenges.
Molecular Techniques for HIV and STDs: Implicationsfor Research and Disease Control in the New Millenium
Rosanna W. Peeling, David C. W. Mabey, and King K. Holmes