Placenta and Trophoblast: Methods and Protocols, Volume I

Placenta and Trophoblast: Methods and Protocols, Volume I

Hardcover(2006)

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Overview

Placenta research has progressed rapidly over the past several decades by taking advantage of technical advances, such as microarray analysis, reverse transcriptase polymerase chain reaction, protein analysis, and in situ hybridization. In Placenta and Trophoblast: Methods and Prools, Volumes 1 & 2, internationally recognized investigators describe cutting-edge laboratory techniques for the study of the trophoblast and placental biology. The techniques presented range from experimental animal models, to animal and human placental organ and cell culture systems, to morphological, biochemical, and molecular strategies for assessing trophoblast/placental growth, differentiation, and function. Volume 1 provides readily reproducible prools for studying embryo-uterine implantation, trophoblast cell development, and the organization and molecular characterization of the placenta. Highlights include strategies for the isolation and culture of trophoblast cells from primates, ruminants, and rodents, and precise guidance to the molecular and cellular analysis of the placental phenotype. A companion second volume concentrates on methods for investigating placental function.

Comprehensive and state-of-the-art, Placenta and Trophoblast: Methods and Prools, Volumes 1 & 2 provide researchers a firm foundation for successful cellular and molecular analysis of the placenta and the establishment of pregnancy.

Editorial Reviews

Doody's Review Service

Reviewer: Bruce A. Fenderson, PhD (Thomas Jefferson University)
Description: The mammalian embryo leaves its free-floating aquatic life shortly after fertilization and embeds itself in the uterine wall. Uterine endometrial tissue provides a necessary substrate for further development, and the developing placenta begins to regulate maternal-fetal exchange of blood gasses, nutrients, and immunological factors. This book introduces a wide array of modern laboratory protocols for studying the biology of the placenta. This is an exciting area of research with important implications for maternal health and fetal development. The book includes 31 chapters that provide detailed methods for studying the placenta in a variety of mammalian species. Examples of interesting research topics in Volume I of this two-book set include immortalization of endometrial cells, induction of trophoblast from embryonic stem cells, propagation of trophoblast stem cells, and microarray analysis of trophoblast cells. The principal focus of each chapter is on cell biology, biochemistry, and molecular biology.
Purpose: This book is part of the popular Methods in Molecular Medicine series, which provides detailed research methods, as well as tips and trouble-shooting advice. According to the editors, the aim of these two volumes is to offer contemporary approaches for studying the biology of the placenta. Volume I addresses protocols regarding mechanisms of implantation and placental development. Volume II continues with protocols for studying functions of the placenta, including regulation of maternal immune response and cellular adaptations to disease.
Audience: It is written for basic science and clinical researchers in mammalian embryology. Graduate students, medical students, research fellows, and established investigators will enjoy reading this book to broaden their horizons and gain insights into useful approaches for studying the origins, fate and functions of extra-embryonic tissues. Educators will find this book useful for illustrating state-of-the-art methods for studying embryogenesis. Biologists will appreciate the cross-species comparisons and multidisciplinary approach. The authors are experts in the field.
Features: This compact book comes with a companion CD that contains color illustrations. Each chapter includes a brief summary, list of key words, introduction, and complete listing of references. The line drawings, illustrations, photomicrographs are well done. Notes appended to each chapter enable the authors to provide explanations, tips, and suggestions for conducting successful experiments. Data generated from the authors' laboratories are used to illustrate key techniques.
Assessment: This book provides step-by-step instructions for conducting research in mammalian embryology. The contributions are well-written and user friendly. The methods and references are up-to-date. The chapters provide helpful introductions, reviews of methodology, and summaries of key experimental findings. The theoretical and practical importance of this research, and the diversity of topics presented, will help this book capture the attention of a wide audience of readers. The techniques are time-tested and so the book will provide an excellent laboratory resource for years to come. One suggestion I have for the authors of chapters in protocol books is that they should summarize the key questions under investigation. Too often, the basis for interest in a particular topic is assumed without explanation. This is an important contribution to the literature, and I recommend it for all those interested in learning more about maternal-fetal interactions during pregnancy.

Product Details

ISBN-13: 9781588294043
Publisher: Springer-Verlag New York, LLC
Publication date: 11/01/2005
Series: Methods in Molecular Medicine , #121
Edition description: 2006
Pages: 470
Product dimensions: 6.10(w) x 9.25(h) x 0.04(d)

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