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Cold Spring Harbor Laboratory Press
Manipulating the Mouse Embryo: A Laboratory Manual / Edition 3

Manipulating the Mouse Embryo: A Laboratory Manual / Edition 3


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Regarded as the "Bible" of mammalian embryo manipulation techniques since the 1986 publication of the first edition of Manipulating the Mouse Embryo: A Laboratory Manual, the third edition of this essential laboratory resource has now been completely reorganized, rewritten, and updated by a new cast of authors. The result is a compilation of new, cuttingedge protocols that include embryonic stem cell production and genetic manipulation, mouse chimeras, mouse cloning, assisted reproduction strategies (including intracytoplasmic sperm injection and in vitro fertilization), whole embryo culture systems, electroporation, embryo and gamete cryopreservation and rederivation, and gene expression, as well as more extensive background information on the use of these techniques. The "gold standard" techniques for applying recombinant DNA technology to investigations of mammalian embryonic development included in the first two editions of this book are also updated and recast, as is the summary of the current state of understanding of mouse development at the molecular level. This book is the premier authoritative and comprehensive source of technical and theoretical guidance for mouse developmental biologists and geneticists and is an essential resource for newcomers to these fields.

Product Details

ISBN-13: 9780879695910
Publisher: Cold Spring Harbor Laboratory Press
Publication date: 12/13/2002
Edition description: Quark
Pages: 776
Product dimensions: 8.42(w) x 11.08(h) x 1.24(d)

Table of Contents

Chapter 1. Developmental Genetics and Embryology of the Mouse: Past, Present, and Future
Chapter 2. Summary of Mouse Development
Chapter 3. Production of Transgenic and Chimeric Mice: General Issues
Chapter 4. Recovery and In Vitro Culture of Pre-implantation-Stage Embryos
Chapter 5. Isolation, Culture, and Manipulation of Post-implantation Embryos
Chapter 6. Surgical Procedures
Chapter 7. Production of Transgenic Mice
Chapter 8. Isolation, Culture, and Manipulation of Blastocyst-derived Stem Cells
Chapter 9. Vector Designs for Embryonic Stem Cell-based Transgenesis and Genome Alterations
Chapter 10. Introduction of Foreign DNA into Embryonic Stem Cells
Chapter 11. Production of Chimeras
Chapter 12. Detection and Analysis of Mouse Genome Alterations and Specific Sequences
Chapter 13. Parthenogenesis, Pronuclear Transfer, and Mouse Cloning
Chapter 14. Assisted Reproduction: Ovary Transplantation, In Vitro Fertilization, Artificial Insemination, and Intracytoplasmic Sperm Injection
Chapter 15. Cryopreservation, Rederivation, and Transport of Mice
Chapter 16. Techniques for Visualizing Gene Products, Cells, Tissues, and Organ Systems
Chapter 17. Setting up and Equipping a Micromanipulation Lab

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