| Owen Wilson | Lightning McQueen |
| Larry the Cable Guy | Mater |
| John Turturro | Francesco Bernoulli |
| Eddie Izzard | Miles Axlerod |
| Michael Caine | Finn McMissile |
| Emily Mortimer | Holly Shiftwell |
| Bonnie Hunt | Sally |
| Tony Shalhoub | Luigi |
| Cheech Marin | Ramone |
| Jason Isaacs | Siddeley,Leland Turbo |
| Joe Mantegna | Grem |
| Peter Jacobson | Acer |
| Thomas Kretschmann | Professor Z |
| Guido Quaroni | Guido |
| Lloyd Sherr | Fillmore,Combat Ship |
| Paul Dooley | Sarge |
| John Ratzenberger | Mack |
| Jenifer Lewis | Flo |
| Michael Wallis | Sheriff |
| Katherine Helmond | Lizzie |
| Franco Nero | Uncle Topolino |
| Vanessa Redgrave | The Queen,Mama Topolino |
| Bruce Campbell | Rod "Torque" Redline |
| Michel Michelis | Tomber |
| Jeff Gordon | Jeff Gorvette |
| Darrell Waltrip | Darrell Cartrip |
| Lewis Hamilton | Lewis Hamilton |
| David Hobbs | David Hobbscap |
| Brent Musburger | Brent Mustangburger |
| Jeff Garlin | Otis |
| Sig Hansen | Crabby |
| Patrick Walker | Mel Dorado |
| Teresa Gallagher | Mater's Computer |
| Stanley Townsend | Victor Hugo,Vladimir Trunkov,Ivan the Tow Truck |
| Velibor Topic | Alexander Hugo |
| John Mainieri | J. Curby Gremlin |
| Brad Lewis | Tubbs Pacer |
| Edie McClurg | Minny |
| Richard Kind | Van |
Technical Credits
| John Lasseter | Director,Original Story |
| Sharon Calahan | Cinematographer |
| Dan Fogelman | Original Story |
| Michael Giacchino | Score Composer |
| Harley Jessup | Production Designer |
| Jeremy Lasky | Cinematographer |
| Brad Lewis | Original Story |
| Natalie Lyon | Casting |
| Tom MacDougall | Musical Direction/Supervision |
| Jake Martin | Production Manager |
| Jake Martin | Production Manager |
| Tom Myers | Sound/Sound Designer |
| Mark Nielsen | Associate Producer |
| Ben Queen | Screenwriter |
| Denise Ream | Producer |
| Kevin Reher | Casting |
| Stephen Schaffer | Editor |
